The 3D Cell Culture & Tissue Engineering
Featured Products
- Swine Skeletal Muscle Fibroblasts
- Swine Pancreatic Islets Cells
- Swine Lung Alveolar Cells
- Swine kidney Fibroblasts
- Swine Hepatocytes
- Swine Dermal Fibroblats
- Swine Cardiomyocytes
- Swine Cardiac Fibroblasts
- Rat Sprague Dolly Serum Wistar
- Rat Sprague Dolly Serum SD
- Rat Sprague Dolly Serum Immuno-deficient
- Rat Sprague Dolly Plasma Pooled Wistar
- Rat Sprague Dolly Plasma Pooled SD
- Rat Sprague Dolly Plasma Pooled Immuno-deficient
- Rat Sprague Dolly Plasma Female Immuno-deficient
- Rat Schwann Cells Wistar
- Rat Schwann Cells SD
- Rat Schwann Cells Immuno-deficient
- Rat Pulmonary Fibroblasts Wistar
- Rat Pulmonary Fibroblasts SD
- Rat Pulmonary Fibroblasts Immuno-deficient
- Rat Lymphatic Fibroblasts Wistar
- Rat Lymphatic Fibroblasts SD
- Rat Lymphatic Fibroblasts Immuno-deficient
- Rat IGS Serum Wistar
- Rat IGS Serum SD
- Rat IGS Serum SD
- Rat IGS Serum Immuno-deficient
- Rat IGS Plasma Wistar
- Rat IGS Plasma SD
- Rat IGS Plasma Pooled Wistar
- Rat IGS Plasma Pooled SD
- Rat IGS Plasma Pooled Immuno-deficient
- Rat IGS Plasma Immuno-deficient
- Rat Hepatocytes Suspension Wistar
- Rat Hepatocytes Suspension SD
- Rat Hepatocytes Suspension Immuno-deficient
- Rat Hepatocytes Plateable-Wistar
- Rat Hepatocytes Plateable-SD
- Rat Hepatocytes Plateable-Immuno-deficient
- Rat Cardiomyocytes Wistar
- Rat Cardiomyocytes SD
- Rat Cardiomyocytes Immuno-deficient
- Rat Cardiac Fibroblasts Wistar
- Rat Cardiac Fibroblasts SD
- Rat Cardiac Fibroblasts Immuno-deficient
- Rat Brain Vascular Pericytes Wistar
- Rat Brain Vascular Pericytes SD
- Rat Brain Vascular Pericytes Immuno-deficient
- Rat Bone Marrow Derived NK Cells Wistar
- Rat Bone Marrow Derived NK Cells Wistar
- Rat Bone Marrow Derived NK Cells Immuno-deficient
- Rat Bone Marrow Derived Muse Cells Wistar
- Rat Bone Marrow Derived Muse Cells SD
- Rat Bone Marrow Derived Muse Cells Immuno-deficient
- Rat Bone Marrow Derived Muse Cells
- Rat Bone Marrow Derived Mononuclear Cells Wistar
- Rat Bone Marrow Derived Mononuclear Cells Immuno-deficient
- Rat Bone Marrow Derived Mononuclear Cells
- Rat Bone Marrow Derived Mesenchymal Stem Cells Wistar
- Rat Bone Marrow Derived Mesenchymal Stem Cells SD
- Rat Bone Marrow Derived Mesenchymal Stem Cells Immuno Deficient
- Rat Bone Marrow Derived Dendritic Cells Wistar
- Rat Bone Marrow Derived Dendritic Cells SD
- Rat Bone Marrow Derived Dendritic Cells Immuno-deficient
- Primary Human Hepatic Stellate Cells
- Mouse Primary Bone Marrow Derived NK Cells CD1
- Mouse Primary Bone Marrow Derived NK Cells C57
- Mouse Plateable Hepatocytes (CD1)
- Mouse Plateable Hepatocytes (BalbC)
- Mouse NOD SCID Plasma
- Mouse NOD SCID Lung Microsomes Mixed Gender
- Mouse NOD SCID Liver S9 Fraction Mixed Gender
- Mouse NOD SCID Liver Microsomes Mixed Gender
- Mouse NOD SCID Intestinal S9 Fraction Mixed Gender
- Mouse NOD SCID Intestinal Microsomes Mixed Gender
- Mouse NOD SCID Intestinal Cytosol Mixed Gender
- Mouse Muse cells CD1
- Mouse Muse cells C57
- Mouse Muse cells BalbC
- Mouse Lung S9 Fraction Mixed Gender
- Mouse Lung Microsomes Mixed Gender
- Mouse Lung Lysosomes Mixed Gender
- Mouse Lung Cytosol Mixed Gender
- Mouse Liver S9 Fraction Mixed Gender
- Mouse Liver Microsomes Mixed Gender
- Mouse Liver Microsomes Mixed Gender
- Mouse Liver Lysosomes Mixed Gender
- Mouse Liver Cytosol Mixed Gender
- Mouse Intestinal S9 Fraction Mixed Gender
- Mouse Intestinal Microsome Mixed Gender
- Mouse Intestinal Lysosomes Mixed Gender
- Mouse Intestinal Cytosol Mixed Gender
- Mouse Hybrid Plasma
- Mouse Hybrid Lung S9 Fraction Mixed Gender
- Mouse Hybrid Lung Microsomes Mixed Gender
- Mouse Hybrid Lung Lysosomes Mixed Gender
- Mouse Hybrid Lung Cytosol Mixed Gender
- Mouse Hybrid Liver S9 Fraction Mixed Gender
- Mouse Hybrid Liver Microsomes Mixed Gender
- Mouse Hybrid Liver Lysosomes Mixed Gender
- Mouse Hybrid Liver Cytosol Mixed Gender
- Mouse Hybrid Intestinal S9 Fraction Mixed Gender
- Mouse Hybrid Intestinal Microsomes Mixed Gender
- Mouse Hybrid Intestinal Lysosomes Mixed Gender
- Mouse Hybrid Intestinal Cytosol Mixed Gender
- Mouse Hepatocytes Suspension CD1
- Mouse Hepatocytes Suspension C57
- Mouse Hepatocytes Suspension BalbC
- Mouse Hepatocytes Plateable C57
- Mouse Derived Mesenchymal Stem Cells
- Mouse Derived Dendritic Cells
- Mouse DBA S9 Fraction Mixed Gender
- Mouse DBA Plasma
- Mouse DBA Lung S9 Fraction Mixed Gender
- Mouse DBA Lung Microsomes Mixed Gender
- Mouse DBA Lung Lysosome Mixed Gender
- Mouse DBA Lung Cytosol Mixed Gender
- Mouse DBA Liver S9 Fraction Mixed Gender
- Mouse DBA Liver Lysosomes Mixed Gender
- Mouse DBA Liver Cytosol Mixed Gender
- Mouse DBA Intestinal Microsomes Mixed Gender
- Mouse DBA Intestinal Lysosomes Mixed Gender
- Mouse DBA Intestinal Cytosol Mixed Gender
- Mouse Cytosol Mixed Gender
- Mouse Cardiomyocytes CD1
- Mouse Cardiomyocytes C57
- Mouse Cardiomyocytes BalbC
- Mouse Cardiac Fibroblasts CD1
- Mouse Cardiac Fibroblasts C57
- Mouse Cardiac Fibroblasts BalbC
- Mouse C57 BL/6N Plasma
- Mouse C57 BL/6N Lung S9 Fraction Mixed Gender
- Mouse C57 BL/6N Lung Microsomes Mixed Gender
- Mouse C57 BL/6N Lung Lysosomes Mixed Gender
- Mouse C57 BL/6N Lung Cytosol Mixed Gender
- Mouse C57 BL/6N Liver S9 Fraction Mixed Gender
- Mouse C57 BL/6N Liver Microsomes Mixed Gender
- Mouse C57 BL/6N Liver Lysosomes Mixed Gender
- Mouse C57 BL/6N Liver Cytosol Mixed Gender
- Mouse C57 BL/6N Intestinal S9 Fraction Mixed Gender
- Mouse C57 BL/6N Intestinal Microsomes Mixed Gender
- Mouse C57 BL/6N Intestinal Lysosomes Mixed Gender
- Mouse Brain Vascular Pericytes
- Human Umbilical Cord Blood Derived NK cells
- Human Umbilical Cord Blood Derived Mononuclear cells
- Human Umbilical Cord Blood Derived Dendritic Cells
- Human Umbilical Cord Blood Derived CD34+ Cells
- Human T Helper Cells
- Human Splenic Fibroblasts
- Human Splenic Endothelial Cells
- Human Skin S9 Fraction Mixed Gender
- Human Skin Derived Microvascular Dermal Endothelial Cells Adult
- Human Skin Derived Epidermal Melanocytes Fetal
- Human Skin Derived Epidermal Melanocytes Adult
- Human Skin Derived Epidermal Keratinocytes Neonatal
- Human Skin Derived Epidermal Keratinocytes Fetal
- Human Skin Derived Epidermal Keratinocytes Adult
- Human Skin Derived Dermal Fibroblasts Fetal
- Human Skin Derived Dermal Fibroblasts Adult
- Human Serum Peripheral Blood Single Donor
- Human Serum Cord Blood Single Donor
- Human Serum Bone Marrow Single Donor
- Human Seminal vesicles microvascular endothelial cells
- Human Seminal Vesicles Fibroblasts
- Human Seminal Vesicles Endothelial cells
- Human S9 Fraction Heart
- Human S9 Fraction
- Human Pulmonary Small Airway Epithelial Cells
- Human Pulmonary Fibroblasts
- Human Pleatable Hepatocytes Pooled
- Human Plateable Hepatocytes
- Human Plasma Cord Blood Pooled
- Human Plasma
- Human Peripheral Blood-Derived NK Cells
- Human Peripheral Blood-Derived Muse Cells
- Human Peripheral Blood-Derived Mononuclear Cells
- Human Peripheral Blood-Derived Monocytes
- Human Peripheral Blood-Derived Mesenchymal Stem Cells
- Human Peripheral Blood-Derived Cytotoxic T-Cells
- Human Pericardial Fibroblasts
- Human Ovarian Surface Epithelial Cells
- Human Ovarian Fibroblasts
- Human Muse cells
- Human Microvascular Endothelial Cells
- Human Mammary Smooth Muscle Cells
- Human Mammary Fibroblasts
- Human Mammary epithelial cells
- Human Lung S9 Fraction Mixed Gender
- Human Lung Microsomes Mixed Gender
- Human Liver S9 Fraction Mixed Gender
- Human Liver Microsomes Mixed Gender
- Human Liver Microsomes
- Human Kidney Fibroblasts
- Human Islet Beta Cells
- Human Intestine Microsomes Pooled Mixed Gender
- Human Intestinal S9 Fraction Mixed Gender
- Human Hepatocytes in Suspension
- Human Eye Derived Limbal Fibroblasts
- Human Extra Embryonic Fetal Tissues Derived Mesenchymal Stem Cells
- Human Extra Embryonic Fetal Tissues Derived CD34 Positive Cells
- Human Endometrial Epithelial Cells
- Human Dental Pulp Derived Mesenchymal stem cells
- Human Dental Pulp Derived Gingival Fibroblasts
- Human Cytotoxic T Cells
- Human Cardiomyocytes
- Human Cardiac Fibroblasts
- Human Bronchial Fibroblasts
- Human Bone Marrow-Derived NK Cells
- Human Bone Marrow-Derived Mononuclear cells
- Human Bone Marrow-Derived Mesenchymal Stem Cells
- Human Bone Marrow-Derived Dendritic cells
- Human Bone Marrow-Derived CD 34 positive cells
- Human Aortic Smooth Muscle Cells
- Human Aortic Endothelial Cells
- Human Adipose Tissue-Derived Stromal Vascular Fraction
- Human Adipose Tissue-Derived Preadipocytes
- Human Adipose Tissue derived Mesenchymal Stem cells
Drop your Query
Ever since a technique of growing cells in an artificial environment has been established, it has evolved with no leaps and bounds. Today, different techniques of cell culture have been routinely used to understand different properties of cells and their applications. Amongst them, 3D cell culture is currently trending for its advanced and convenient features available as compared to their alternatives. Moreover, 3D cell culture is expected to solve some mysteries related to in vitro organ cultures, opening altogether new dimensions of organ replacement techniques as compared to conventional organ transplants.
At Kosheeka we are advancing our knowledge and expertise to support this exclusive journey of in vitro organ culture.
As all of us may already be aware, currently different techniques available for 3D cell culture; while each of them offers different positives and a few negatives. Before moving ahead to understand them, it is always better to first compare 3D cell culture with its conventional counterpart, the 2D technique.
3D cell culture technique is vastly privileged to facilitate cellular differentiation and tissue organization using micro-assembled, custom-designed structures, supported by a complex microenvironment. Many studies have vetoed that cells in 3D culture are very sensitive toward morphological as well as physiological changes; which not only influence genetic expression but also enhance cellular communication.
With the help of 3D cell culture, researchers can grow two different cellular populations together, which are known as cocultures and can exactly reproduce the cellular functions observed within a tissue.
Thus, unlike 2D culture, 3D culture is a relatively new technique and requires a lot of understanding, expertise, and supportive accessories like different 3D culture matrices. However, for various applications 3D cell culture is a more satisfactory model for mimicking in vivo cell behaviors and organizations. While assembling multi-layered 3D cell culture requires optimum supporting microenvironment influencing cellular morphology, and differentiation potential to the great extent.
As discussed above, scaffolds can be used as a convenient support for 3D cell culture techniques. The flow of oxygen, nutrients, and waste products largely depend upon the porosity of the scaffolds. The more porous the scaffold is, the more cellular proliferation and migration within the web of the scaffold; and they eventually adhere to the same. While cells keep growing, the mature cells interact with each other and will eventually turn into structures close to the tissue they initially originated from.
As discussed, depending upon the type of cells to be handled, at Kosheeka we have invented adequate scaffolds possessing suitable properties and shapes. These scaffold layouts match the tissue of interest while reproducing its structure, scale, and functions.
Some of our scaffolds that have gained good recognition are:
- Hydrogel scaffolds
- Nongel polymer scaffolds
- Lyophilized membranes
- The recombinant matrices
Conclusively, biomaterials are gaining promising positions in the field of tissue engineering and translational applications.
Recently, organoids generated from primary cells, especially tissue-specific stem cells are identified to be the ideal candidates for reproducible and scalable 3D models for organs on chips. These organoids have expressed similar properties during in vitro culture as that of stem cells, and originated from specific tissue; some of these properties can be listed as self-renewal, differentiation properties, property of adherence, etc. These models address different limitations of existing models including:
- Similar composition and architecture as that of primary tissues
As they harbor a small population of self-renewing stem cells, further differentiating into cells of all major lineages, with comparable properties and frequencies as in physiological conditions.
- Relevant models of in vivo conditions
These organoids are more biologically relevant to any other organ system and are identified to manipulate niche components and gene sequences.
- Stable system for extended cultivation
These organoids can be cryopreserved for a longer duration as biobanks and expanded indefinitely by leveraging self-renewal, differentiation capability of stem cells, and intrinsic ability to self-organize.
As stated above, these organoids are produced either with primary cells or with other pluripotent stem cells like embryonic stem cells and/or induced pluripotent stem cells by appropriate physical and biochemical cues. The physical cues for cellular attachment and survival are collagen, fibronectin, entactin, and laminin; while biochemical cues are EGF, FGF-10, R-spondin, WNT3A, etc.
Out of various known applications of organoids, some of the applications mentioned herewith are holding great promise in both basic research and translational medicines.
These applications are:
- Developmental Biology
- Disease pathology of infectious diseases
- Regenerative Medicine
- Drug toxicity and efficacy testing
- Personalized medicine
The products of Kosheeka supporting organoids formation are
- Organoid Dissociation Medium
- Organoid Freezing Medium
- Organoid Conditioning Medium
These 3D balls formed due spatial arrangement of primary cells during in vitro cultures are critically important in the current biomedical practices. These proliferative spheres were named in the 1970s when hamster lung cells were grown in the culture, during which they arranged themselves into perfect spheres. Studies have indicated that these spheroids can increase cellular connectivity, and facilitate more rigorous communication for understanding the cellular microenvironment; which can in turn offer realistic tissue models for studying different pathophysiology.
The applications of spheroids are especially encouraging in regenerative medicine research, cancer research, and during drug screening.
Despite their great requirements, currently one of the primary challenges in their production is to find primary cells that are robust, consistent, proliferative, and mimic the exact tissue of isolation. In fact, the latter is very important for achieving physiologically relevant outcomes because the cellular functions of the spheroids show a correlation to the size of the cell cluster.
Kosheeka is on the verge of developing a range of products supporting the production of spheroids like
- Primary cells and/or stem cells including cancer stem cells
- Serum-free growth medium for optimum proliferation and growth
- GMP compliant