Primary Hepatocytes in Suspension CD-1

We offer transporter qualified hepatocytes in suspension format, customized as per your specifications like single donor/pooled/male/female/mixed gender. These specialized cells are ideal for studying drug transport and metabolism, providing valuable insights into drug interactions and liver related research.

Available formats:
Cryopreserved: Cryogenic vial containing ≥ 500000 viable cells

+ Product Sheet
+ MSDS
+ PI

General Description Specifications Certificate Of Analysis Technical Library

We provide cryopreserved suspension hepatocytes that are thoroughly characterized for high viability and general phase I and phase II metabolism activities in short-term suspension metabolic pathways. Each batch undergoes extensive quality control procedures, and detailed reports can be provided upon request.

These transporter qualified suspension hepatocytes are ideally suited for short-term in vitro drug clearance assays, with a recommended usage time of less than 4 hours. However, it's important to note that these suspension hepatocytes are not suitable for assays that require adherent cells.

These pre-characterized cells offer a cost-effective and straightforward test system for in vitro metabolite identification and studies related to metabolic stability. Each cell lot is accompanied by a proper Certificate of Analysis, ensuring the highest quality standards for your research needs.

Product Category	Mus musculus, Mouse CD-1
Product Type	Suspension culture
Derived From	Liver tissues
Cell Morphology	Non adherent, spheroidal suspension culture
Culture & Growth Properties	Non-adherent population
Passage No.	P1
Mycoplasma	Detected on demand
Hepatitis B	Negative
Hepatitis C	Negative
HIV-1	Negative
Aerobic & Anaerobic Bacteria	Negative after 7 days of culture
Positive for	Transporter qualified
Negative for

Suspension hepatocytes have a limited life span compared to plateable hepatocytes, typically lasting a few days to a week in culture. For long-term experiments, fresh isolations may be required to replenish in the culture.

Suspension hepatocytes do not adhere to surfaces in culture, unlike plateable hepatocytes that are optimized for attachments and growth on cell culture vessels Suspension hepatocytes are commonly used for drug development studies and metabolism studies along with liver-specific functional assays, while plateable hepatocytes can be maintained in culture for longer durations.

Suspension hepatocytes are commonly used for drug metabolism studies, enzyme activity assays, and liver specific functional assays. They are also valuable for evaluating drug induced toxicity and liver specific responses in vitro.

Suspension hepatocytes provide a more physiologically relevant environment compared to traditional 2D cultures. They allow for the assessment of liver-specific functions and responses in a more native-like setting.

Transporter-qualified hepatocytes are used in IND-enabling preclinical drug development studies to evaluate the induction potential of compounds for specific cytochrome P450 enzymes, such as CYP1A2, CYP2B6, CYP3A4. These evaluations are crucial to meet regulatory expectations.

You can contact Kosheeka on our direct number i.e., +91 9654321400 or mail us your requirements at info@kosheeka.com and we will get back to you for your queries.

On a generalized note, suspension hepatocytes are used in drug metabolism studies, enzyme activity assessments, and liver-specific functional assays. They are also valuable for evaluating drug-induced toxicity and liver-specific assessments.

Hepatocytes are highly temperature-sensitive cells. To maintain their viability and ensure accurate results, it is crucial to use proper transportation methods, such as liquid nitrogen instead of dry ice to prevent temperature fluctuations during transport.

Yes, we offer various customization options to meet your specific requirements and research needs.

You will receive a detailed Certificate of Analysis with the products. However, for raw data required for publications, a non-disclosure agreement must be signed, acknowledging Kosheeka as your cell supplier.
Please feel free to reach us for any further inquiries or assistance with your research.