Human Bone Marrow-Derived Dendritic cells

Available Format: ≥1000000 cells cryopreserved in suitable medium along with DMSO.

General Description Specifications Certificate Of Analysis Technical Library

Dendritic cells act as immune messengers and are primarily involved in processing antigens derived from pathogens. The dendritic cells are also responsible for presenting these pathogens to T cells of the immune system, to initiate an immune response.Kosheeka is isolating normal human dendritic cells from bone marrow, isolated from the ileum of the crest after local anaesthesia. The cells are further analyzed for a panel of surface markers like CD11c+, CD86+, CD80+, HLADR+, and CD14-.It should be noted that these cells cannot survive in the culture media for more than seven days. For seven days, they can be maintained in culture as immature cells with the addition of proper cytokines like IL-4 and GM-CSF. For further differentiation of dendritic cells into mature immune cells, they can be incubated with specific cytokines like TNF-α and IL-1β.These specific cells can be primarily used in antigen presentation and studies associated with understanding the uptake of drug-induced immunosuppression due to cytokine secretion.

Product Category	Homo Sapiens, Human
Product Type	Suspension cell population
Derived From	Bone Marrow
Cell Morphology	Huge, granular lymphocytes
Culture & Growth Properties	Non-adherent, proliferating population
Passage No.	Customized
Mycoplasma	Detected on demand
Hepatitis B	Negative
Hepatitis C	Negative
HIV-1	Negative
Aerobic & Anaerobic Bacteria	Negative after 7 days of culture
Positive for	CD 11C+, CD86+, HLA DR+
Negative for	CD 14-

These dendritic cells are isolated from human bone marrow-derived mononuclear cells. These mononuclear cells are a fraction of a mixed population of cells, including monocytes, macrophages, lymphocytes, etc.

When dendritic cells are isolated from the tissue source, they are further purified and placed in a nutritive culture medium. However, it should be noted that in normal circumstances, these cells cannot be proliferated in a cell culture medium; on the contrary, they die more rapidly than expected. To stop this and increase their viability, it is suggested to add certain cytokines like IL-4 and GM-CSF in a concentration of

The maturity of dendritic cells is assessed through high-throughput cellular imaging like AFM or scanning electron microscopy. The mature dendritic cells are clearly visible as long, delicate figures; extending from the cell bodies in an outward direction.

Dendritic cells are found in the blood and other tissues that are exposed to the environment, like skin, the lining of the nose, the lungs, and the stomach. Their primary role is to adjust to the innate and adaptive immune responses as per environmental stimulus. Dendritic cells are also responsible for generating resistance during allergy, autoimmunity, and transplant rejection.