Primary Cells: Lifestyle Disease Models
Primary cells, isolated from different donor tissues along with paired media for optimal cell growth and characterization are currently in demand, especially for pharmaceutical drug discovery efforts. These disease models are specifically designed to address various lifestyle diseases, including but not limited to autoimmune disorders, inflammatory disorders, skin injuries, etc. Some of these cellular disease models include cardiovascular disorders, lung disorders, arthritis, and diabetes.
With the availability of primary cells from specific tissue, it is now possible to understand the disease pathophysiology at the cellular and molecular levels. With the creation of relevant experimental models’ researchers are trying to mimic similar physiological conditions that are thought to be essential in developing new therapeutic modalities for chronic diseases. Additionally, disease models created using primary cells can be used for assessing clinical outcomes, and drug screening purposes.
Depending upon our client’s requirements, Kosheeka is happy to offer our unique range of primary cells that are customized as per demand. Choose from our wide range of inventory and start your experiments immediately.
Traditionally, research around the world was supported by cancer cell lines. These cell lines were the most preferred tools for all in vitro experimental studies, as well as the disease model of choice. Today, biomedical research has advanced crossing all the boundaries, with many impressive scientific accomplishments and a plethora of new insights into disease mechanisms. While these advancements have certainly helped the scientific community to bring forth new treatment modalities, they also outline the persistent challenges as well as limitations that are being faced in translational research; such as the use of inadequate disease models due to the unavailability of specific cell lines, cell line contamination as well as cell line misidentification. These issues are further complicating the risky, costly, and time-consuming nature of drug screening.
With the major leap in the era of translational medicine, the biomedical field is getting equipped with reliable resources for faster and more affordable personalized treatments. In the race against primary cells and the use of immortalized cell lines, the latter is largely reconsidered.
Accordingly, Kosheeka has supported the creation of different ‘disease models’ created using our wide portfolio. The disease models created using a single type of primary cells are helpful in better understanding due to the reduction in complexity of the disease; hence, these models have become easy to explore. With the easy availability of primary cells and the ability to produce them on a mass scale, we can provide cells to you as per your requirements.
Accordingly, some of the extensively used primary cells for creating different disease models are:
- Primary Human Bronchial Epithelial Cells for Lung Disorders
- Primary Human Skin fibroblasts for Wound healing
- Primary Human Cardiomyocytes for Cardiovascular Disorders
- Primary Human lung Fibroblasts for Asthma
- Primary Human Preadipocytes for Diabetes Type II
- Primary Human Adipose Tissue-Derived Mesenchymal Stem cells for Diabetes Type II
- Complete growth medium for optimum growth and maintenance.
Diabetes Type II Disease Model
All of us are aware of the growing incidences of obesity, causing higher mortality due to important pathological consequences, like Diabetes Type II. With current statistical analysis, by 2030; developed countries like America would be handling approximately 350 million individuals with more attribution of expenditure, estimated to be around 132 million $ in the US alone.
Studies have indicated that progressive deterioration of adipogenic potential of preadipocytes, present in adult human bodies may importantly contribute to the adipose tissue dysfunction leading to obesity and type 2 diabetes (T2D). Various studies are also proposing variable epigenomic signatures as well as transcriptomic responses at the cellular level to be responsible for the differentiation pattern; hence, in vitro disease models were developed using primary human preadipocytes to study transcriptome profiles, epigenetic signatures sourced from lean, obese and obese with Type 2 Diabetes patients.
Asthma Cellular Disease Model
Similarly, in disease models like asthma research and drug development; various primary cells from bronchial, and tracheal tissues of human origin are commonly used. This can also be a way out to avoid regulatory obligations in case of animal usage. The models created with the help of primary cells are still considered benchmarks; despite them being high in cost, difficult to be available, and difficult to maintain in culture for a longer period. Whereas, various freshly isolated primary cells from bronchial or airway tissues like human bronchial epithelial cells, etc. under appropriate culture conditions, may get differentiated into pseudostratified monolayers of mixed epithelial cells, further being considered as the best possible in vitro representation of bronchial epithelium, as confirmed by some of the studies.
Some of the common primary cells, used as a disease model in respiratory illnesses including Asthma are
- Primary Human Aortic Endothelial Cells
- Primary Human Bronchial Epithelial Cells
- Primary Human Coronary Artery Endothelial Cells
- Primary Human Coronary Artery Smooth Muscle Cells
- Primary Human Carotid Artery Endothelial Cells
- Primary Human Lung Microvascular Endothelial Cells
- Primary Human Tracheal Epithelial Cells
