Cancer Research The Modern Research Tool

Considering the wide prevalence of cancer, various cancer cell lines are routinely used as the modern in vitro model systems for cancer research and drug discovery. Their applications in the scientific world are primarily linked with the use of an easily available replica of in vivo growing cancer for experimental purposes through the indefinite source of biological material. The world of medical research has evolved over the period, and the cell lines isolated from cancer cells are frequently used in biomedical research for understanding the disease pathophysiology; to explore potential new treatments. These immortalized cell lines grow continuously in an unlimited number of passages.

Kosheeka is contributing to cancer research through several cell lines as a viable alternative that is free from any contamination.

Cancer cell lines are perfect in vitro model systems used as a potential alternative to study biological processes. These cell lines are prepared with immortalized cancer cells that divide and grow continuously under optimum laboratory conditions. This goes without explaining why human cancer cell lines are more deliberately used to study cancer biology for authenticating existing treatments and developing efficient ones. Currently, multiple areas of ongoing research are accompanying CRISPR as a method to correct pathogenic point mutations in these cell lines.

However, it is very important to understand the basic difference between primary cancer cells and cancer cell lines, which one is the better tool for cancer research as well as gene editing techniques like CRISPR.

Cancer cell lines vs primary cancer cells: what is the difference?

There is a cardinal difference between immortal cell lines and cancer cell lines and is associated with their genetic makeup. Studies have revealed so far that genetic instability is the key feature associated with the cancerous proliferation of cells, and the same has been proven based on genetic heterogeneity observed in tumour biopsies. Interestingly, this heterogeneity is one of the reasons for tumour recurrence. Contradictory to the same, immortal cell lines are typically dominated by one clone of prospective tumour cells, further lacking heterogeneity. Some studies have even suggested that these cancer cell lines are majorly prone to genetic drift, when cultured for a longer duration, and hence are not true responses to cancers. Technologically as well, due to recent advances in CRISPR, it is quite feasible to study genetic drifts in primary cells as opposed to cancer cell lines. However, one should understand that a wide range of studies going on with both primary cells and cancer cell lines.

Kosheeka is maintaining a good inventory for different types of cancer like

  • Leukaemia cell lines

– HL-60

  • Pancreatic cancer cell lines

– PANC-1

  • Colorectal cancer cell lines

– CACO-2

  • Prostate cancer cell lines

– DU 145

  • Liver cancer cell lines

– HEP G2

  • Breast cancer cell lines

– HCC 1937

The cancer cell lines have a long-lasting history to study underlying molecular mechanisms, especially in some fields like cardiovascular diseases and neuroscience. Some of the important advantages to choose cancer cell lines as disease models are that they carry the number of aberrant mutations that arose in the tumour, from which these cell lines were derived. Importantly, these cancer cell lines are used to study many biological processes that have been widely used in pharmacological studies. Recently, OMICS technology has supported the characterization of hundreds of other cell lines; which further reinforced the concept of cell line usefulness in cancer biology. These data have been made available through multiple online datasets, which further made it possible to identify detailed molecular alterations and mutations. This way, it is possible for researchers to select an appropriate model system for their research. At the same time, we encourage the right use of cell lines, following appropriate guidelines along with the best quality and consistency.

Considering the challenges that may be encountered in developing a suitable model, including requisites like special media, extracellular matrices and finally the healthy, cancer cell lines/cancer stem cells to develop the model correctly.

On a path to developing the right cancer model for your experiments, Kosheeka may help you to

  • To obtain the right starting model i.e., cancer stem cells or cancer cell lines that thoroughly reflect tumour biology. We can even help you in providing patient-specific customized primary stem cells for your experiments.
  • To provide special extracellular matrices that mimic the cellular and matrix complexity of native tissues.
  • Large scale production of definitive cells and cell lines to reduce batch variation and inconsistency.