Over the decades, cell lines have played a significant role in scientific advancements. Nonetheless, due to some drawbacks of cell lines, scientists had to be extremely attentive while handling them. But here comes the game changer- Primary Cells! Primary cells are isolated directly from the tissues of humans or animals and are developed under optimal...
Mesenchymal stem cells (MSCs) are multipotent adult stem cells. Being mutipotent, MSCs can differentiate into more than one specialized cell of the body. Generally, MSCs make the different cells in skeletal tissues e.g., chondrocytes, osteoblast, adipocyte etc. In case of MSCs giving rise to other specialized cells like neurons, reprogramming of the cells with gene...
Biomedical research advancements depend on data credibility but scientific results are not always reproducible. Independent verification of cell culture findings is a basic principle of research and the procedure and other factors affect the concept of research reproducibility heavily. Many scientists who work with primary cell culture focus on the ‘correctness’ of the results but...
The vasculature tissue consists of different types of cells: smooth muscle cells, endothelial cells, connective tissue cells, and pericytes (Johnson et al., 2002). Pericytes were discovered by Charles Marie Benjamin Rouget, a French physiologist, 140 years ago (Herndon et al., 2017). Pericytes are present in every vascularized body tissue and cover 22 to 99 %...
ADME is the abbreviation for Absorption, Distribution, Metabolism, and Excretion. These ADME studies are designed to investigate the metabolism and processing of a chemical compound by a living organism. When toxicology tests are a part of this process, the acronym becomes ADMET with the last T for ‘Toxicology’. Primary cells are directly isolated from human...
Primary cells mimic the tissue of origin as they are directly isolated from the tissue and processed for culturing under optimized media conditions. Therefore, primary cell culture provides excellent model systems for studying normal cell physiology and biochemistry. There are a lot of articles on primary cells vs cell lines to understand which one of them...
Cell culture is a prominent tool in cellular and molecular biology research, often spreading to clinical applications. Cell culture using primary cells can be excellent model systems for studying physiology and biochemistry of cells, drug toxicity and metabolism, and other biomedical applications. Primary cell culture is being used by researchers worldwide for their role in...
Primary cells can undergo a pre-determined and finite number of cell divisions in culture. With more passage in cell culture, primary cells enter a replicative senescence state, where morphology, gene expression, and metabolism alter. Therefore, scientists immortalize primary cells in vitro to study cell growth, differentiation, and senescence using continuous cell lines. Immortal cell lines made from primary...
In the field of biomedical research, 2D cell cultures have extensively been used since the early 1900s but in recent times, the technology of 3D cell culture has boomed. The tussle of 2D vs 3D cell culture has received much interest from biomedical researchers and other science geeks. In this article, let us explore more...
In biomedical research and development, scientists mostly use cultured cell lines as models because they offer easy, inexpensive, and stable platforms. However, cell lines fail to grasp the microenvironment physiology that occurs in vivo. Human primary cells come to the rescue in this situation! Primary cell culture portrays the complex physiological cell behavior and therefore...
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