Diabetes is a global concern for the healthcare community. It is a chronic metabolic disorder that can either be Type1 (failure to produce enough insulin) or Type2 (inability of the body to use insulin). The exact cause of Type1 Diabetes is still under research and is believed to be an autoimmune disorder with the involvement...
A crucial step before taking up research work with human or animal tissue is to ensure appropriate ethical and medical legislation and guidelines for experiments. This can also deal with procuring approval from relevant authorities or individuals. Biohazards and Safety considerations It is very important to know the possible associated risks while working with potential...
Primary cells mimic the tissue of origin as they are directly isolated from the tissue and processed for culturing under optimized media conditions. Therefore, primary cell culture provides excellent model systems for studying normal cell physiology and biochemistry. There are a lot of articles on primary cells vs cell lines to understand which one of them...
Cell culture is a prominent tool in cellular and molecular biology research, often spreading to clinical applications. Cell culture using primary cells can be excellent model systems for studying physiology and biochemistry of cells, drug toxicity and metabolism, and other biomedical applications. Primary cell culture is being used by researchers worldwide for their role in...
Primary cells represent the in vivo tissue environment fairly and these cells are directly taken from body tissues for processing and established them under optimized culture conditions. As these cells are derived directly from native body tissue and not modified, they mimic the in vivo state and physiology. Therefore, they provide excellent model systems for...
The growth of normal human mammary epithelial cells, including luminal, myoepithelial, and/or basal cells, is tightly controlled. Mammary epithelial cells grow for a finite span and eventually die or undergo senesce. Human, rat, and murine mammary epithelial cell have provided evidence that essential initial steps in mammary carcinoma cell line growth involve the loss of...
The task of choosing cell culture media for use with your primary cells is surely daunting. Therefore, if you are thinking of replicating a formulation of media from any previously published report or if you are optimizing your nutrient or classical media, it is important to understand the consequences of this media choice for your...
Primary cell culture is an expanding technology for several biomedical applications like tissue culture, 3D Cell Culture, bioscaffolds, 3D bioprinting, organoid culture etc. Researchers have been utilizing this technology as an efficient ethical alternative to animal experiments and as a physiologically in vivo-mimicking alternative to culturing continuous cell lines. We, at Kosheeka, believe that cell...
If you are a cell culture researcher working with primary cell culture, the most difficult thing to accept is watching your cell dishes and flasks getting contaminated or not proliferating even with appropriate incubation at the correct media pH and temperature conditions. Watching those adherent cells not attached to the flask surface and knowing that...
Cryopreservation is an essential cell culture maintenance activity in biomedical labs to preserve cells for future use. There are various aspects of cryopreservation which need to be learnt and properly performed so as to obtain a good viable population of cells after thawing, in case a culture follows. Keeping these things in mind, we had...
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