Primary cells mimic the tissue of origin as they are directly isolated from the tissue and processed for culturing under optimized media conditions. Therefore, primary cell culture provides excellent model systems for studying normal cell physiology and biochemistry. There are a lot of articles on primary cells vs cell lines to understand which one of them...
Cell culture is a prominent tool in cellular and molecular biology research, often spreading to clinical applications. Cell culture using primary cells can be excellent model systems for studying physiology and biochemistry of cells, drug toxicity and metabolism, and other biomedical applications. Primary cell culture is being used by researchers worldwide for their role in...
The growth of normal human mammary epithelial cells, including luminal, myoepithelial, and/or basal cells, is tightly controlled. Mammary epithelial cells grow for a finite span and eventually die or undergo senesce. Human, rat, and murine mammary epithelial cell have provided evidence that essential initial steps in mammary carcinoma cell line growth involve the loss of...
The task of choosing cell culture media for use with your primary cells is surely daunting. Therefore, if you are thinking of replicating a formulation of media from any previously published report or if you are optimizing your nutrient or classical media, it is important to understand the consequences of this media choice for your...
Primary cell culture is an expanding technology for several biomedical applications like tissue culture, 3D Cell Culture, bioscaffolds, 3D bioprinting, organoid culture etc. Researchers have been utilizing this technology as an efficient ethical alternative to animal experiments and as a physiologically in vivo-mimicking alternative to culturing continuous cell lines. We, at Kosheeka, believe that cell...
If you are a cell culture researcher working with primary cell culture, the most difficult thing to accept is watching your cell dishes and flasks getting contaminated or not proliferating even with appropriate incubation at the correct media pH and temperature conditions. Watching those adherent cells not attached to the flask surface and knowing that...
Cryopreservation is an essential cell culture maintenance activity in biomedical labs to preserve cells for future use. There are various aspects of cryopreservation which need to be learnt and properly performed so as to obtain a good viable population of cells after thawing, in case a culture follows. Keeping these things in mind, we had...
A good biomedical research paper writing (review or research paper) requires an intensive literature search for information pertaining to the study, whether it involves primary cell culture or microbial studies. This literature survey takes into account rigorous searching and compiling of all previous studies and data on a particular topic. The literature survey helps further...
Antibiotics have been a routinely used component in cell cultures to prevent the growth of bacteria but several researchers doing primary cell culture have showed their interest in avoiding them for several reasons in order to save their cultures and help the cells grow happy! Researchers unanimously agree on the saying, “With good lab practice,...
In vitro cell culture systems have been considered as essential research tools for biomedical and clinical research and development. Although it started with 2D cell culture consisting of cell monolayers, the recent practice of cell culture, specifically for primary cell culture, advanced 3D in vitro culture systems are widely being adopted for the advantages of...
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