Cryopreservation is an essential cell culture maintenance activity in biomedical labs to preserve cells for future use. There are various aspects of cryopreservation which need to be learnt and properly performed so as to obtain a good viable population of cells after thawing, in case a culture follows. Keeping these things in mind, we had...
Organoids are ex vivo model systems that revolutionized basic and translational cancer research in the domain of disease biology, since last decade. Organoid 3D cultures undergo mitogen-mediated proliferation as multicellular structures in semisolid matrices and while they possess some similarities like 3D spheroid cultures of cell lines, there are basic fundamental differences between them (Tsai...
A good biomedical research paper writing (review or research paper) requires an intensive literature search for information pertaining to the study, whether it involves primary cell culture or microbial studies. This literature survey takes into account rigorous searching and compiling of all previous studies and data on a particular topic. The literature survey helps further...
Antibiotics have been a routinely used component in cell cultures to prevent the growth of bacteria but several researchers doing primary cell culture have showed their interest in avoiding them for several reasons in order to save their cultures and help the cells grow happy! Researchers unanimously agree on the saying, “With good lab practice,...
At the time of procuring new primary cells to start cell culture for research purposes, the decision of procuring fresh cells or frozen primary cells (cryopreserved cells) may not be a simple task. There are many factors that goes on to influence the decision, based oncell maintenance, cell type, cell functionality, and logistics. To help...
Humans have “used” animals not only as a source of food but also for companionship, recreation and transport for ages. According to (CULABBR, 1988), scientific research has used a wide range of animal usage in labs, such as guinea pigs, hamsters, rats, mice, fish, birds, cats, dogs and primates. The research conducted on animals covers...
There is a medical condition involving the backflow or retrograde flow of the contents of the stomach to the larynx and pharynx to contact the upper aero-digestive tract. This condition is called Laryngopharyngeal reflux (LPR). Research by El-Serag in Clinical Gastroenterology and Hepatology showed that the occurrence of such acid reflux conditions has shot up...
In order to minimize problems of misidentification that can lead to research being retracted and the loss associated due to contamination. The following pointers can be used as a reminder to facilitate good practices for primary cultures in biomedical research: The derivation of culture from fresh human tissue involves the first step of obtaining ethical...
If you are looking at your laptop screen and thinking about your Ph.D. supervisor telling you: “Why don’t you start putting your data together in a publication format so we can get it through to an international journal?”. It is high time to focus on the process of compiling your scientific data and get going...
3D cell culture has been a boon to the scientific community for cell culture experiments and is extensively used as an alternative to in vivo animal experiments. 3D cell culture presents itself as an assembly of cells that mimics tissue-specific / organ-specific architecture. 2D cell culture is the most common cell culture practice in vitro...
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