Cryopreservation has become one of the major steps to focus on enhancing commercial and clinical cellular experiment-based cell culture process. Non-optimized substandard protocols and reagents used in cell culture can lead to damage of cells and lower cell viability. Besides that, the lack of optimized protocols in cell culture practice might also lead to decreased...
Primary cell culture is an expanding technology for several biomedical applications like tissue culture, 3D Cell Culture, bioscaffolds, 3D bioprinting, organoid culture etc. Researchers have been utilizing this technology as an efficient ethical alternative to animal experiments and as a physiologically in vivo-mimicking alternative to culturing continuous cell lines. We, at Kosheeka, believe that cell...
If you are a cell culture researcher working with primary cell culture, the most difficult thing to accept is watching your cell dishes and flasks getting contaminated or not proliferating even with appropriate incubation at the correct media pH and temperature conditions. Watching those adherent cells not attached to the flask surface and knowing that...
Cryopreservation is an essential cell culture maintenance activity in biomedical labs to preserve cells for future use. There are various aspects of cryopreservation which need to be learnt and properly performed so as to obtain a good viable population of cells after thawing, in case a culture follows. Keeping these things in mind, we had...
Antibiotics have been a routinely used component in cell cultures to prevent the growth of bacteria but several researchers doing primary cell culture have showed their interest in avoiding them for several reasons in order to save their cultures and help the cells grow happy! Researchers unanimously agree on the saying, “With good lab practice,...
At the time of procuring new primary cells to start cell culture for research purposes, the decision of procuring fresh cells or frozen primary cells (cryopreserved cells) may not be a simple task. There are many factors that goes on to influence the decision, based oncell maintenance, cell type, cell functionality, and logistics. To help...
Cell culture started in the 1800s and with time, the trend of exploring new domains of cell culture technology to excel in biomedical and clinical research has been expanding into newer domains. Although 2020 has majorly focused the utilization of animal cell culture in COVID-19 diagnosis and drug development research besides playing an essential role...
In order to minimize problems of misidentification that can lead to research being retracted and the loss associated due to contamination. The following pointers can be used as a reminder to facilitate good practices for primary cultures in biomedical research: The derivation of culture from fresh human tissue involves the first step of obtaining ethical...
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