Liver Derived Primary Cells

Primary Human Hepatic Stellate Cells

Ethically sourced from liver tissues, stellates are the specialized cell types; primarily responsible for storing retinoids. However, in response to liver damage, they undergo a transformation, losing their stored retinoids and becoming proliferating fibroblast like cells. These activated stellate cells deposit collagen matrix, leading to the accumulation of collagen in the liver, a condition known as liver cirrhosis.In order to study stellate cells, they can be isolated from liver tissues; where they are enriched in their population and can be cultivated further in culture. Once in culture, stellate cells immediately differentiate into their activated state, characterized by high expression of alpha-smooth muscle actin (alpha-SMA).At Kosheeka, we are isolating stellate cells from liver tissues and are growing them in culture with the help of specialized media. These cells are mainly characterized for the stellate cell morphology after cryopreservation and thawing. The expression of vimentin is specifically checked to understand the quality of the cells isolated; this protein is primarily assessed with the help of flow analysers. We can also perform immunohistochemical experiments on demand, for the presence of alpha smooth muscle actin, a key marker of activated stellate cells.

Human Plateable Hepatocytes

Models for drug-to-drug interactions (DDI) with more clinical relevance is a serious concern for any new drug development project. The plateable hepatocytes are specialized primary liver cells, isolated from liver tissues of different species; depending upon customer requirements. These cells display a unique property of being able to adhere and grow on culture plates or dishes. These cells are used as indispensable tools for researchers in drug development, toxicology, disease modeling, as well as liver-related studies.At Kosheeka, these cells are tested for high viability and healthy culture duration. The cells isolated at Kosheeka support the multiple potential of DDI, as they are induction qualified. The cells are characterized for 3 major mechanisms:

• Transporter activity
• Enzyme activity

These cells are featured for their confluence monolayer, distinct morphology with cobblestone appearance. Inducibility of enzyme activity for CYP3A4, CYP2B6, and CYP1A2. Basal metabolism for 8 CYPs, SULT, UGT and aldehyde oxidase are analyzed for the product. These cells are pretested for long term culture, spheroid formation, and 96-well compatibility.
Application

• Xenotransplantation
• Low clearance metabolism studies
• Disease modeling
• Xenotransplantation
• 3D cell culture
• Short term cellular toxicity studies