Dental Pulp Mesenchymal Stem Cells

Dental pulp stem cells are a type of mesenchymal stem cells that have shown great promise in treating stomatognathic disorders. Due to their success at preclinical as well as clinical levels, they are the preferred research tools, especially in vitro research pertaining to maxillofacial and periodontal disorders. The adult dental pulp stem cells (DPSCs) are isolated with ethical consent from the third molars of the adult person; during the extraction of wisdom teeth. During characterization, it was observed that these DPSCs express similar structural and functional markers as that of mesenchymal stem cells, isolated from other well-known sources, like wharton’s jelly, bone marrow, etc. Similar to the MSCs isolated from other sources, DPSCs also exhibit transdifferentiation potential to chondrogenic, adipogenic, and osteogenic lineages.  The DPSCs can be expanded up to ten passages to express markers like CD105, CD90, CD73 and are found to be negative for markers like CD34 and CD45. Cells are tested to be negative for mycoplasma, bacteria, yeast and fungi. On demand, cell extract is also tested for HIV-1, Hepatitis C and Hepatitis B.Each lot of cells comes with a detailed certificate of analysis.  

Gingival Fibroblasts

Kosheeka obtains Human Gingival Fibroblasts from the gingival tissues of individuals who've given informed consent, particularly from patients dealing with periodontal issues. These fibroblasts possess a unique ability related to scarless wound healing. To retain their therapeutic potential, we culture these cells using explant cultures, ensuring the preservation of their receptors' integrity. These fibroblasts are passed up to stage P1 and undergo specific characterization. We confirm the presence of fibroblast markers CD29 and CD73 while ensuring the absence of the mesenchymal stem cell marker CD105. The cells are also assessed for their in vitro differentiation capacity to adipogenic, osteogenic as well as chondrogenic differentiation potential.For therapeutic purposes, we offer testing for infectious markers such as HIV, HCV, and HBV upon request. Regular assessments also include screenings for aerobic, anaerobic bacteria, and mycoplasma infection. To meet diverse needs, these cells are shipped either cryopreserved in vials containing 1 million cells or supplied in an active state within T75 flasks.