Blood Derived Stem Cells

Peripheral Blood-Derived Muse Cells

Human peripheral blood-derived multilineage-differentiating stress-enduring (Muse) cells are a type of adult stem cells that were first identified in human bone marrow and have also been found in peripheral blood. Muse cells are unique in that they have the ability to self-renew and differentiate into multilineage cell origin, including neural, muscles and endothelial cells, without formation of any new kind of tumor.Peripheral blood derived Muse cells can be isolated from a very small volume of peripheral blood using a simple protocol. The isolation method involves collecting peripheral blood through apheresis and separating the mononuclear fraction of isolated samples using differential centrifugation. The mononuclear cell fraction is then subjected to mechanical stress, which induces muse cells to enter the suspension. Muse cells can then be isolated based on their ability to adhere to the plastic surface and form colony-like structures. These cells can then be expanded in the culture, under appropriate conditions using suitable cocktail of cytokines and other growth factors. Due to their ability to eradicate the risk of tumor development and maintain low telomerase activity even in the stressed conditions, they are a front-runner for therapeutic applications as well as IVD and toxicity analysis.Muse cells are typically identified and characterized for several distinctive markers including CD105 expression, stage-specific embryonic antigen-3, etc. These cells are also distinguished from the other adherent population with the help of their low proliferation rate as well as spontaneous differentiation capacity including neurons, adipocytes as well as hepatocytes.

Human Peripheral Blood-Derived NK Cells

The natural killer cells are a type of lymphocytes, primarily involved in regulation of the immune system. They are called “Natural” because they don’t require prior activation in order to recognize abnormal and/or infected cells to lyse them. These cells are also able to recognize and kill cells that have downregulated or lost major histocompatibility complex (MHC) on their surface, which can occur in cancer cells and/or virus infected cells. These cells are also involved in secretion of cytokines, which are signalling molecules that help to coordinate and regulate immune response. These cells are found in the blood, lymph nodes and spleen.In humans, NK cells represent around 8-20% of the circulating lymphocytes. Unlike other lymphocytes, NK cells do not express antigen-specific receptors. NK cells play a critically important role in regulating adaptive immune response. Studies are ongoing to understand the role of NK cells in activation of cytokines post viral infections. Due to their ability to recognize tumor cells, NK cells are widely studied as primary components in immune-oncology.Kosheeka has validated isolation protocol for peripheral blood derived NK cells using positive immunomagnetic separation. However, we have also validated other methods of isolation of NK cells like differential centrifugation or fluorescence activated cell sorting; depending upon experimental demands. Kosheeka NK cells are traditionally characterized for the presence of CD56 cell surface markers and the absence of CD3. Due to separation of cells through immunomagnetic selection, the isolated cells express highest purification. All the donors are tested for infectious markers.