BalbC Dendritic cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

BalbC Mesenchymal stem cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

BalbC Mononuclear cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

BalbC Muse cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

BalbC NK cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

C57 Dendritic cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

C57 Mesenchymal stem cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

C57 Mononuclear cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

C57 Muse cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

C57 NK cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

CD-1 Dendritic cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

CD-1 Mesenchymal stem cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

CD-1 Mononuclear cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

CD-1 Muse cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

CD-1 NK cells

Available formats: ≥ 1.0 x 106/ ml. cryopreserved in suitable medium

Dendritic cells

Available formats: ≥ 2.5 x 106/ ml. cryopreserved in suitable medium

Human Muse cells

Multi-lineage differentiating stress enduring cell (MUSE Cell) is a subpopulation of Umblical Cord MSC’s. Unlike other types of stem cells hey are able to differentiate into all three germ layers spontaneously or under cytokine induction. Muse cells are unique because of their low telomerae activity which eradicates the risk of oncogenisis. This makes them a front-runner for therapeutic applications as well as IVD and toxicity analysis.

Human Peripheral Blood-Derived Cytotoxic T-Cells

The human peripheral blood derived cytotoxic T cells, also known as the CD8+ T cells and cytotoxic T cells, are a kind of T lymphocytes that play a very important role in protecting us from invading infectious agents and cancer through regulation of immune system. These cells are a subset of T lymphocytes, the key players of our immune system helping us in recognizing and protecting from foreign invaders by destroying them.

Most cytotoxic T cells express T-cell receptors (TCRs), which are helpful in recognizing a specific glycoprotein and an antigen called as CD8, binding with the constant portion of the class I MHC.  Upon binding, these cells release certain cellular cytotoxins like perforin, granzymes as well as granulysin that are responsible for triggering cascade of signalling reactions promoting apoptosis. This important property makes them important tool for understanding pathophysiology of many viral diseases including hepatitis B and inflammatory diseases like rheumatoid arthritis. Moreover, these cells have also shown promising results in the treatment of certain cancers like melanoma, leukaemia, etc.

The peripheral blood derived cytotoxic T cells are obtained from patient’s own samples and can be expanded upon activation using a cocktail of cytokines and other growth factors. Kosheeka recommends use of IL-2 to activate T cells that are further purified based on the expression of surface marker CD8, which is the hallmark of cytotoxic T cells. The final product is further tested for purity, viability and function to ensure that it meets the necessary quality standards for clinical applications.

The Kosheeka CD8+ T cells are isolated from peripheral blood from healthy donor, using negative immunomagnetic selection.

Human Peripheral Blood-Derived Mesenchymal Stem Cells

Human peripheral blood-derived mesenchymal stem cells are a type of stem cells obtained from the peripheral blood of healthy donors. These cells are the multipotent population with the ability to differentiate into cells of various tissue origin, like bone, cartilage, fat, and muscle cells. The population of cells is currently highly exploited in the field of regenerative medicine. The cells are being investigated for use in various therapeutic applications, including bone and cartilage repair, wound healing, and treatment of inflammatory and autoimmune diseases. One of the advantages of PB-MSCs is their ease of isolation from a non-invasive source, as compared to other counterparts like bone marrow. Their higher proliferation rate makes them a valuable tool for other clinical applications as well. Kosheeka is isolating a pure population of peripheral blood-derived mesenchymal stem cells from a mononuclear fraction of peripheral blood, using a process called density gradient centrifugation. Once, isolated these cells can further be expanded in culture and characterized for various markers like CD105, CD 29, CD73, etc. On-demand, Kosheeka can also help to purely isolate mesenchymal stem cells through the negative selection method. These cells demonstrate a unique ability to differentiate into adipogenic, chondrogenic, and osteogenic lineages; when cultured in a definite differentiation medium. All cells test negative for mycoplasma, bacteria, yeast, and fungi. All our donors are screened for infectious diseases, and a detailed certificate of analysis is provided for each cell lot purchased. 

Human Peripheral Blood-Derived Monocytes

Human peripheral blood derived monocytes are a type of white blood cells that originate in bone marrow and circulate in the bloodstream. These monocytes play a very important role in the development of the innate immune system and are responsible for phagocytosis, or the engulfing and destruction of pathogens. They are also responsible for presenting antigens to T cells to activate the adaptive immune response. Research is ongoing to understand their role in viral pathogenesis and development of inflammatory diseases like rheumatoid arthritis, atherosclerosis, etc. Studies are ongoing to investigate the molecular mechanism by which monocytes are recruited to the site of inflammation and the factors that are responsible for regulation of macrophages and dendritic cells. Their role in cancer immunotherapies has also been recently explored, in order to evaluate their potential role in the development of cancer vaccines. Kosheeka Human Peripheral Blood Derived Monocytes are isolated from peripheral blood of screened, healthy, untreated donors. Briefly, mononuclear cells are isolated from apheresis derived peripheral blood through differential centrifugation methods. The population of mononuclear cells is further purified through positive immunomagnetic selection directed against CD14. These cells are further characterized for the expression of CD14 markers. The donor blood is also screened for infectious markers like HIV, HCV, HBsAg and HBcAg. 

Human Peripheral Blood-Derived Mononuclear Cells

The peripheral blood-derived mononuclear cells are a cocktail of potent progenitors along with immune cells like NK cells, T cells, and B cells. Additionally, the cocktail is also a rich source of monocytes that are destined to be differentiated into macrophages and/or dendritic cells. The cells are isolated using the kit with the help of density gradient separation. All the red blood cells are thoroughly depleted by proprietary techniques with osmotic lysis. Although the cellular profiles of mononuclear cells are mostly donor-dependent, on average, they may contain around 50% T cells, 20% monocytes, 10% B cells, and 10% NK cells. The collection is from healthy donors, who are screened for infectious markers like HIV-1, Hepatitis B, and Hepatitis C. Each lot shall be provided with a Certificate of Analysis. Kosheeka is able to demand a viable population of freshly isolated mononuclear cells. These cells will be transported in HBSS along with 5 mM EDTA and 0.5% BSA. We recommend the use of any suitable hematopoietic growth medium from a reputed brand, along with a combination of growth factors/cytokines, depending upon the study requirements and applications. 

Human Peripheral Blood-Derived NK Cells

The natural killer cells are a type of lymphocytes, primarily involved in regulation of the immune system. They are called “Natural” because they don’t require prior activation in order to recognize abnormal and/or infected cells to lyse them. These cells are also able to recognize and kill cells that have downregulated or lost major histocompatibility complex (MHC) on their surface, which can occur in cancer cells and/or virus infected cells. These cells are also involved in secretion of cytokines, which are signalling molecules that help to coordinate and regulate immune response. These cells are found in the blood, lymph nodes and spleen.In humans, NK cells represent around 8-20% of the circulating lymphocytes. Unlike other lymphocytes, NK cells do not express antigen-specific receptors. NK cells play a critically important role in regulating adaptive immune response. Studies are ongoing to understand the role of NK cells in activation of cytokines post viral infections. Due to their ability to recognize tumor cells, NK cells are widely studied as primary components in immune-oncology.Kosheeka has validated isolation protocol for peripheral blood derived NK cells using positive immunomagnetic separation. However, we have also validated other methods of isolation of NK cells like differential centrifugation or fluorescence activated cell sorting; depending upon experimental demands. Kosheeka NK cells are traditionally characterized for the presence of CD56 cell surface markers and the absence of CD3. Due to separation of cells through immunomagnetic selection, the isolated cells express highest purification. All the donors are tested for infectious markers.

Mouse Muse cells BalbC

Multi-lineage differentiating stress enduring cell (MUSE Cell) is a subpopulation of Umblical Cord MSC’s. Unlike other types of stem cells hey are able to differentiate into all three germ layers spontaneously or under cytokine induction. Muse cells are unique because of their low telomerae activity which eradicates the risk of oncogenisis. This makes them a front-runner for therapeutic applications as well as IVD and toxicity analysis.

Mouse Muse cells C57

Multi-lineage differentiating stress enduring cell (MUSE Cell) is a subpopulation of Umblical Cord MSC’s. Unlike other types of stem cells hey are able to differentiate into all three germ layers spontaneously or under cytokine induction. Muse cells are unique because of their low telomerae activity which eradicates the risk of oncogenisis. This makes them a front-runner for therapeutic applications as well as IVD and toxicity analysis.