Islets cells are the major part of pancreas. The beta cells constitute the predominant type of cell in the islets. The beta cells are particularly important because they make insulin. Degeneration of the beta cells is the main cause of type I (insulin-dependent) diabetes mellitus.
| Organism | Sus scrofa, Swine (Domestic Pig) |
| Tissue | Pancreas |
| Cell Type | Islets |
| Morphology | Spherical; Epithelial like when in adherent culture |
| Culture and Growth Properties | Suspension cell population |
| Passage No at the time of cryopreservation | Freshly isolated Beta cells have a limited lifespan in culture and should only be thawed immediately prior to their intended use. |
| Cells per Vial | Minimum 2.5 x107 cells |
| Sterility Test | Negative for Bacterial, Mycoplasma, Yeast |
| Viability | ≥ 70 % on revival from cryopreserved state* |
| Biosafety Level | Minimum BSL-1 containment.
Kosheeka recommends proper safety procedures as described in all scientific literature to be undertaken when handling primary cells. Although primary cells isolated from non-diseased donors are not known to cause disease in healthy adult humans. Animal Material Precaution All tissues used for isolation are obtained after Animal ethical clearance. Although, tissue collection is from healthy animals, we recommend that all cells be accorded the same level of biosafety consideration as cells known to carry animal born infections. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome. |
| Disease | Normal |
| Age | Adult |
| Gender | Lot Specific |
| Ethnicity | lot-specific |
| Applications | β cell biology, facilitate drug discovery, and provide a pathway to β cell replacement therapy for the treatment of diabetes. |
| Product Format | frozen 1 mL |
| Storage Conditions | -130°C or below |
Catalogue Number: HM-1302
Catalogue Number: MB-3101
Catalogue Number: SN- 2951
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