Hyperglycemia promotes epithelial-mesenchymal-transition and stem cell properties in pancreatic ductal epithelial cells. Pancreatic ductal epithelial cells are utmost importance in studies related to pancreatic cancer.
| Organism | Homo sapiens, human |
| Tissue | pancreas, duct |
| Cell Type | Epithelial cell |
| Morphology | Epithelial like, cuboidal morphology. |
| Culture and Growth Properties | Adherent cell population |
| Passage No at the time of cryopreservation | Second Passage The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency. |
| Cells per Vial | 5 x105 cells |
| Sterility Test | Negative for Bacterial, Mycoplasma, Yeast |
| Viral Testing | Hepatitits B: negative Hepatitis C: Negative HIV 1: Negative HIV 2: Negative |
| Viability | ≥ 70 % on revival from cryopreserved state* |
| Biosafety Level | Minimum BSL-1 containment.
Kosheeka recommends proper safety procedures as described in all scientific literature to be undertaken when handling primary cells. Although primary cells isolated from non-diseased donors are not known to cause disease in healthy adult humans. Human Material Precaution All tissues used for isolation are obtained under informed consent and conform to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome. |
| Disease | Diabetes |
| Age | Adult |
| Gender | lot-specific |
| Ethnicity | lot-specific |
| Applications | Assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications. |
| Product Format | frozen 1 mL |
| Storage Conditions | -130°C or below |
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