Dermal fibroblasts are cells within the dermis layer of skin which are responsible for generating connective tissue and allowing the skin to recover from injury. Using organelles, dermal fibroblasts generate and maintain the connective tissue which unites separate cell layers.
| Organism | Mus musculus (Mouse) |
| Tissue | Skin |
| Cell Type | Fibroblast |
| Morphology | Spindle Shaped elongated cells |
| Culture and Growth Properties | Adherent cell population |
| Passage No at the time of cryopreservation | Second passage |
| Cells per Vial | Minimum 2.5 x105 cells |
| Sterility Test | Negative for Bacterial, Mycoplasma, Yeast |
| Viability | ≥ 70 % on revival from cryopreserved state* |
| Biosafety Level | Minimum BSL-1 containment.
Kosheeka recommends proper safety procedures as described in all scientific literature to be undertaken when handling primary cells. Although primary cells isolated from non-diseased donors are not known to cause disease in healthy adult humans. Animal Material Precaution All tissues used for isolation are obtained after Animal ethical clearance. Although, tissue collection is from healthy animals, we recommend that all cells be accorded the same level of biosafety consideration as cells known to carry animal born infections. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome. |
| Disease | Normal |
| Age | Juvenile |
| Gender | Lot Specific |
| Stain | Balb C |
| Applications | Response to pathogens, skin aging, wound healing, gene delivery, skin diseases. |
| Product Format | frozen 1 mL |
| Storage Conditions | -130°C or below |
Catalogue Number: MB-3101
Catalogue Number: HB-1202
Catalogue Number: HP-1701
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