Human umbilical vein endothelial cells (HUVECs) are cells derived from the endothelium of veins from the umbilical cord. HUVECs can be easily made to proliferate in a laboratory setting. Like human umbilical artery endothelial cells they exhibit a cobblestone phenotype when lining vessel walls.
| Organism | Homo sapiens, human |
| Tissue | Umbilical cord vein |
| Cell Type | Endothelial Cells |
| Morphology | Cobblestone appearance with large dark nuclei; during proliferation, cells are small and evenly sized, display a high mitotic index and show no presence of smooth muscle cells. |
| Culture and Growth Properties | Adherent cell population |
| Passage No at the time of cryopreservation | Second Passage The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency. |
| Cells per Vial | 5 x105 cells |
| Sterility Test | Negative for Bacterial, Mycoplasma, Yeast |
| Viral Testing | Hepatitits B: negative Hepatitis C: Negative HIV 1: Negative HIV 2: Negative |
| Viability | ≥ 70 % on revival from cryopreserved state* |
| Biosafety Level | Minimum BSL-1 containment.
Kosheeka recommends proper safety procedures as described in all scientific literature to be undertaken when handling primary cells. Although primary cells isolated from non-diseased donors are not known to cause disease in healthy adult humans. Human Material Precaution All tissues used for isolation are obtained under informed consent and conform to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome. |
| Disease | Normal |
| Age | Child / Adult |
| Gender | lot-specific |
| Ethnicity | lot-specific |
| Applications | wound healing, angiogenesis, oxidative stress, Immune response, cellular response to viral or bacterial infection, arteriolosclerosis, drug screening, tubule formation |
| Product Format | frozen 1 mL |
| Storage Conditions | -130°C or below |
Catalogue Number: SP-2701
Catalogue Number: ML-3601
Catalogue Number: SM-2304
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