Human Umbilical Cord Vein Endothelial Cells

Human umbilical cord-derived endothelial cells

Human Umbilical Cord Vein Endothelial Cells

Category: Single/Pooled

Catalogue Number: HU-1902- A

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Description

Human umbilical vein endothelial cells (HUVECs) are cells derived from the endothelium of veins from the
umbilical cord. HUVECs can be easily made to proliferate in a laboratory setting. Endothelial cells are grown in
specifically defined basal media supplemented with different Endothelial cells growth components; in order to
mimic the ideal in vivo cell system. In case of pooled HUVECs, these cells are derived from different donors that
further avoids the variability associated with single donor HUVECs.
We also recommend the use of specific Vascular cell Basal Media (Both options are available with and without
VEGF); in order to ensure the highest viability and plating efficiency of cells.
Specific Culture Requirements:

  1. Endothelial Cell Growth Medium (Cat No: ADC/GM/1902/A) with VEGF containing rh-VEGF, rh-FGF basis, l-
    glutamine, ascorbic acid, fetal bovine serum and a mix of antibiotics for optimum cell growth and viability.
  2. Fibronectin coated flasks for subculturing.

Characteristics

Organism Homo sapiens, human
Tissue Umbilical cord vein
Cell Type Endothelial Cells
Morphology Cobblestone appearance with large dark nuclei; during proliferation, cells are small and evenly sized, display a high mitotic index and show no presence of smooth muscle cells.
Culture and Growth Properties Adherent cell population
Passage No at the time of cryopreservation Second Passage
The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency.
Cells per Vial 5 x105 cells
Sterility Test Negative for Bacterial, Mycoplasma, Yeast
Viral Testing Hepatitits B: negative
Hepatitis C: Negative
HIV 1: Negative
HIV 2: Negative
Viability ≥ 70 % on revival from cryopreserved state*

Specifications

Biosafety Level Minimum BSL-1 containment.

Kosheeka recommends proper safety procedures as described in all scientific literature to be undertaken when handling primary cells. Although primary cells isolated from non-diseased donors are not known to cause disease in healthy adult humans.

Human Material Precaution

All tissues used for isolation are obtained under informed consent and conform to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.
Disease Normal
Age Child / Adult
Gender lot-specific
Ethnicity lot-specific
Applications wound healing, angiogenesis, oxidative stress, Immune response, cellular response to viral or bacterial infection, arteriolosclerosis, drug screening, tubule formation
Product Format frozen 1 mL
Storage Conditions -130°C or below

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