Human umbilical vein endothelial cells (HUVECs) are cells derived from the endothelium of veins from the
umbilical cord. HUVECs can be easily made to proliferate in a laboratory setting. Endothelial cells are grown in
specifically defined basal media supplemented with different Endothelial cells growth components; in order to
mimic the ideal in vivo cell system. In case of pooled HUVECs, these cells are derived from different donors that
further avoids the variability associated with single donor HUVECs.
We also recommend the use of specific Vascular cell Basal Media (Both options are available with and without
VEGF); in order to ensure the highest viability and plating efficiency of cells.
Specific Culture Requirements:

1. Endothelial Cell Growth Medium (Cat No: ADC/GM/1902/A) with VEGF containing rh-VEGF, rh-FGF basis, l-
   glutamine, ascorbic acid, fetal bovine serum and a mix of antibiotics for optimum cell growth and viability.
2. Fibronectin coated flasks for subculturing.