Human Bone Marrow-Derived NK Cells

$435.00

Category:  | SKU: hBM-1204

Human bone marrow derived natural killer cells

Description

The natural killer cells, also commonly known as the NK cells are the lymphocytes or the fighter cells of the immune system; which are primarily recruited by the body as the host defense system. Due to their immune regulatory properties, they are of special interest to researchers as immuno-oncology tools, as these cells can especially target cancer cells or tumor cells and destroy them. Lot of research is currently going on to study ways to inhibit these cells to reduce transplant rejection and failure during IVF.

Human Bone Marrow-Derived NK cells are obtained from the bone marrow of humans, after informed consent. The NK cells are selected from the mononuclear cell fraction of bone marrow either or from the entire bone marrow through the negative immunomagnetic selection method and are characterized for the presence of CD56 cell surface markers.

The Kosheeka Human Bone Marrow-Derived NK cells are positive for CD 56 and CD16 markers and are negative for CD3. The Kosheeka offers an option of choosing your own product depending upon your study requirements.

Available Formats:

Proliferating: ≥500000 viable cells shipped in appropriate growth medium (T25 Flask).

Cryopreserved: A cryogeneic vial containing ≥500000 cells

Additional information

CHOOSE VARIANT

Proliferating, Cryopreserved

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Specifications

Product Category Homo Sapiens, Human
Product Type Adherent cultures, suspension cell population
Derived From Bone Marrow
Cell Morphology Huge, granular lymphocytes
Culture & Growth Properties Adherent, proliferating population
Passage No. Customized
Mycoplasma Detected on demand
Hepatitis B Negative
Hepatitis C Negative
HIV-1 Negative
Aerobic & Anaerobic Bacteria Negative after 7 days of culture
Positive for CD 56, CD16
Negative for CD 3

 

Documents

+ Certificate of Analysis

+ Material Safety Data Sheet

+ Product Sheet

Techinical Library

 Are Kosheeka Human Bone Marrow-Derived NK cells shipped in suspension culture?

We have the option of developing products as per your customized demands. The NK cells derived from human bone marrow can be shipped in both ways, either in suspension or in adherent form; depending upon your requirements. You can also get suspension cultures from us and add an optimum concentration of IL-2 to support their adherence.

Can these cells be allowed to expand and proliferate in culture?

Yes, with the addition of optimum concentration of certain growth factors and cytokines like IL-2, and IL-21; it is possible to expand Human Bone Marrow-Derived NK cells in culture. It is also recommended to us human serum supplemented serum to enhance their adherence properties.

 Do you recommend any medium for the proliferation of NK cells?

Lymphocyte growth medium from any reputed brand is recommended to facilitate the proliferation of NK cells; however, the media should be supplemented with human serum along with suitable cytokines like IL-2, and IL-21.

Can these cells be passaged further or expanded on a mass scale?

No, the isolation process of NK cells decides their cellular outcome. It is not recommended to expand NK cells at a mass scale.

What QC tests are generally performed to assess the quality of NK cells?

The Kosheeka Human Bone Marrow-Derived NK cells are isolated and separated through immunomagnetic sorting. These cells are positive for CD56 and CD16, while negative for CD3. These characterization details can be provided on demand.

 

Downloads

Kosheeka product catalog

Mesenchymal Stem Cells Derived Exosomes for Cosmetic Applications

Application Note: Bone Marrow Mononuclear Cells: Expanding their therapeutic periphery

Bone Marrow Tissue Derived Progenitors