Peripheral blood mononuclear cells are a hetergeneous population of blood cells with a single round nucleus and include macrophages, dendritic cells, monocytes, and lymphocytes.
Homo sapiens, human
spherical; variable after culturing
Culture and Growth Properties
Suspension cell population
Passage No at the time of cryopreservation
Bone marrow derived mononuclear cells have a limited lifespan in culture and should only be thawed immediately prior to their intended use.
Cells per Vial
Minimum 2.5 x107 cells
Negative for Bacterial, Mycoplasma, Yeast
Hepatitits B: negative
Hepatitis C: Negative
HIV 1: Negative
HIV 2: Negative
≥ 70 % on revival from cryopreserved state*
Minimum BSL-1 containment.
Kosheeka recommends proper safety procedures as described in all scientific literature to be undertaken when handling primary cells. Although primary cells isolated from non-diseased donors are not known to cause disease in healthy adult humans.
Human Material Precaution
All tissues used for isolation are obtained under informed consent and conform to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.