Primary Cells
What are Primary cells?
Primary cells are cells derived from living differentiated tissue. Primary cells retain native cellular functions of cells invitro, thus finding its applications in Biomedical, pharmaceutical research etc.
For optimal growth, primary cells require specific media and nutrients in contrast to established cell lines. The uses of primary cells in research have potential to provide more relevant and controlled data because of its resemblance to native cells.
Application of Primary Cells
Drug screening and toxicity evaluation
Primary cells can find its wide application in pharmaceutical industry. Primary cell cultures can be used to evaluate maximum permissible drug dosage and toxicity of drugs.
In vitro model to study Cell biology and metabolic studies
Because of its resemblance to natural and living environment, they can be used as model system to study cell biology, metabolic regulations.
As a substitute for animal studies
Use of primary cell line can reduce the cost of animal experiments.
Tissue Engineering and Regenerative medicine
Currently, researchers are exploring the usage of primary cells in the reconstruction of diseased or damaged tissue or organs.
Advantages of Primary Cells
Closely mimics in vivo conditions
Primary cells closely stimulate biological living model thus provide significant and high quality results in experiments
Minimal chromosomal aberrations
The Major disadvantage with established cell line is that after a period, cell characteristics may change and become quite different from initial passages. Generally primary cells not only retain normal physiology but also genetic constitution when propagated in culture.
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