What are Primary cells?
Primary cells are cells derived from living differentiated tissue. Primary cells retain native cellular functions of cells invitro, thus finding its applications in Biomedical, pharmaceutical research etc.
For optimal growth, primary cells require specific media and nutrients in contrast to established cell lines. The uses of primary cells in research have potential to provide more relevant and controlled data because of its resemblance to native cells.
Culture of Primary Cells
Primary cells are grown after isolation from specific animal tissues. The tissue extracts undergo disaggregation by enzymatic process and are incubated in grown medium for proliferation. Following that, these cells are further purified and separated according to the required cell lineage.
Application of Primary Cells
Drug screening and toxicity evaluation
Primary cells have a wide application in the pharmaceutical industry to evaluate drug dosage and toxicity.
In vitro model for Cell Biology research
Primary cells are excellent in vitro models to study cell biology, biochemical assays, and metabolism.
A substitute for animal studies
Primary cells in 3D cell culture models can bypass the ethical regulations of animal experiments.
Tissue Engineering and Regenerative medicine
Primary cells have extensively applications in tissue engineering and regenerative medicine.
Advantages of Primary Cells
Closely mimics in vivo conditions
Primary cells closely stimulate biological living model thus provide significant and high quality results in experiments
Minimal chromosomal aberrations
The Major disadvantage with established cell line is that after a period, cell characteristics may change and become quite different from initial passages. Generally primary cells not only retain normal physiology but also genetic constitution when propagated in culture.