Modern medicines are technologically advanced commodities. The use of completely automated analysis devices for high throughput screening is becoming more common in drug discovery. Nowadays, magnetic 3D cell culture is coming into light as it offers a more genuine model of human tissue than 2D cell culture and is one interesting high throughput technique. Magnetic 3D culture has the potential to be a useful tool in tumor and therapeutic development. To date, significant progress has been made in the fundamental construction of 3D tumor models, including notable research using 3D tumor cell models to emulate the tumor cells or drug discovery.
Why is 3D cell culture useful in high-throughput screenings?
In vitro 2D test systems are used in several typical high throughput systems. This frequently results in false-positive results, which eventually turn out to be unsuccessful in the human body. To cover the gap between in vitro and human physiology, cancer researchers throughout the world are designing novel 3D preclinical models that more closely reflect the in vivo characteristics of the original tumor in terms of differentiation, variability, structure, and drug responses.
Moreover, since animals do not exhibit human physiology, animal models are often ineffective in assisting with the transition. As a result, the clinical relevance of clinical studies is compromised.
New 3D cell cultures could assist to reduce false positives even more and improve the existing system.
Magnetic 3D cell cultures can be grown in as little as 15 minutes!
The secret to this magnetic approach is that even tiny magnetic forces at the bottom of the wells are enough to regulate cell movement in a certain way. For example, utilizing magnetic levitation, a 3D structure with connected cells that mimics the physiological circumstances of human tissue may be constructed in approximately 15 minutes. This is really fast when compared to many other traditional 3D approaches. The magnetic forces act as an unseen framework, allowing the cells to quickly assemble. The extracellular matrix is instantly expressed by the aggregated cells. Because the bottom of the vessels is not adherent, the cells at the bottom of the wells are held in place by small magnetic pens. These tiny magnetic pens help keep 3D cell cultures in microplates in place.
Advancement in cancer research and personalized drugs development
What makes this approach remarkable is that the size of the spheroids may be controlled based on the number of cells and is not restricted to a single cell type. Primary cells, in particular, provide an excellent opportunity to gain a deeper understanding of specific pathologies. Magnetic 3D cell culture also enables the use of standard 2D cell culture analysis tools, such as cell viability assays, for high throughput screenings.
Researchers can improve their toxicological studies as well as their experimental studies in the field of regenerative medicine by using magnetic 3D cell culture. Above everything, magnetic 3D cell culture has the potential to boost cancer research and speed up the hunt for personalized drugs development. In the future, due to the fact that magnetic levitation in 3D cell culture better mimics tumor biology, it can assist to limit the number of possible therapeutic candidates in the very early stages of drug development. Because this method provides a more precise model of patients’ physiological functions – by using human primary cells or patients’ cells and accurately imitating 3D circumstances; it raises the potential of uncovering viable treatment candidates as early as possible.
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