Of late, immunotherapy has emerged as a powerful technique in the treatment of cancer. Immunotherapy is a kind of cancer treatment that uses the capacity of your own immune system for the elimination and prevention of cancer. The growth of abnormal cells invades the body and leads to cancer. This technique has made commendable changes...
Cancer is one of the deadliest diseases and kills people every now and then. The two most common types of cancer are: Carcinoma and Sarcoma. both of these cancers are malignant but still they have differences. Despite the numerous advancements in tumour and molecular biology over the past few years; the mortality rates due to...
Over the past few decades, cell-based assays have provided scientists with outstanding insights on various cellular processes taking place in the body. Primary cell-based assays have proved to be one of a kind in the field of assay development. Human primary cell assays are developed by extracting cells from blood, differentiated stem cells or bone...
Cell culture is an inseparable part of scientific research and advancements; without primary cells and culture techniques, the scientific progress made so far would have been a dream! Since the time researcher managed to isolate cells from frog nerve fibre in the last century, the science of cell culture has evolved greatly. Today, scientists are...
Mesenchymal stem cells have become popular in terms of clinical interest because of their potential in stem cell transplantation. A lot of clinical trials using mesenchymal stem cells have been achieved but many are still in the pipeline under scrutiny. Mesenchymal stem cell therapy risk should be considered in terms of long-lasting observations instead of...
Cryopreservation is a mandatory technique for cell culture researchers as it allows the storage of cells and tissues in the cryopreserved stage for future culturing. Cryopreservation can impact research efficacy owing to how the whole process is managed and what materials are used. For example, if cells are frozen too quickly, membrane damage and cell...
The process of vaccine production is a continually updating and refreshing technology. The innovative technologies give researchers numerous opportunities to adjust processes and achieve cost-effective scale-up of production. The COVID-19 vaccine rush has shown the world why aligning and optimizing new methods is crucial for companies and organizations. In today’s time, ‘plug and play’ doesn’t...
Primary cells can undergo a pre-determined and finite number of cell divisions in culture. With more passage in cell culture, primary cells enter a replicative senescence state, where morphology, gene expression, and metabolism alter. Therefore, scientists immortalize primary cells in vitro to study cell growth, differentiation, and senescence using continuous cell lines. Immortal cell lines made from primary...
History of Animal Cell Culture Media In 1882, Sydney Ringer developed Ringer’s solution, a balanced salt solution with composition close to that of bodily fluids, to keep beating frog hearts after dissection. This is considered as the first witnessing of in vitro animal tissue cultivation. In the wake of Ringer’s report of the animal Cell...
The vascular endothelium, constituting the interface between organ tissues and blood, is a physical barrier and contributes to several physiological and pathological body functions, for example, thrombosis, angiogenesis, inflammation, metabolite transportation, etc. Hepatic Sinusoidal Endothelial Cells (HSECs) represent 15-20% of liver cells and form the wall of the liver sinusoids. HSECs are highly specialized endothelial...
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