Primary cells represent the in vivo tissue environment fairly and these cells are directly taken from body tissues for processing and establish them under optimized culture conditions. As these cells are derived directly from native body tissue and not modified, they mimic the in vivo state and physiology. Therefore, they provide excellent model systems for studying...
Primary cells can undergo a pre-determined and finite number of cell divisions in culture. With more passage in cell culture, primary cells enter a replicative senescence state, where morphology, gene expression, and metabolism alter. Therefore, scientists immortalize primary cells in vitro to study cell growth, differentiation, and senescence using continuous cell lines. Immortal cell lines made from primary...
History of Animal Cell Culture Media In 1882, Sydney Ringer developed Ringer’s solution, a balanced salt solution with composition close to that of bodily fluids, to keep beating frog hearts after dissection. This is considered as the first witnessing of in vitro animal tissue cultivation. In the wake of Ringer’s report of the animal Cell...
The vascular endothelium, constituting the interface between organ tissues and blood, is a physical barrier and contributes to several physiological and pathological body functions, for example, thrombosis, angiogenesis, inflammation, metabolite transportation, etc. Hepatic Sinusoidal Endothelial Cells (HSECs) represent 15-20% of liver cells and form the wall of the liver sinusoids. HSECs are highly specialized endothelial...
Bone marrow mononuclear cells (BMMCs) are a heterogeneous population of primary cells that are derived from the adult bone marrow. These mononuclear cells include lymphocytes, monocytes, hematopoietic stem cells, and hematopoietic progenitor cells. The bone marrow mononuclear cells also include a population of mesenchymal stromal cells and these cells are isolated from human adult bone...
In recent times, PDX models or patient-derived xenograft models are getting heavily utilized in head and neck oncology research, specifically for studies on radiation response and organoids. What is a Patient-Derived Xenograft? Patient-derived xenografts (PDX) are xenograft models that have been derived from patient tumor tissues but never adapted to grow like cell line-derived xenografts. Patient-derived...
Preclinical research models of cancer, such as human tumor xenografts, murine tumor homografts, cell line models and 3D organoid models, are extensively used in research and development for oncology studies. Since these tumor models are used for a wide spectrum of research applications, they are often utilized globally but the cell lines which are used...
Experiments and assays of your research lead to the establishment of a research paper but the validation of your research work comes only after your research paper is published in an appropriate esteemed journal. Besides validation, publications can extend a wider recognition, promotion, and help in gaining research funds. Unfortunately, publishing is generally easier said...
Pericytes are vascular cells located on capillaries and other small diameter vessels. They have also been reported to be present in the layers of the arteries called intima, media, and adventitia. Their exact functions are yet to be classified. These vascular cells are involved in the development, stabilization, remodeling, and maturation of capillaries and other...
Experiments with primary cells are playing a major role in advancement of life science and pharmaceutical research. Primary cells are little difficult to work with, but these cells provide more meaningful data in experiments. Here are ten common mistakes that are made with primary cells and also how these mistakes can be minimized. These tips will enhance...
We are glad that you preferred to contact us. Please fill our short form and one of our friendly team members will contact you back shortly.
