Once upon a time, the scientific world was struggling to culture the notoriously difficult primary cells; and had to spend a lot of resources, in terms of money as well as time. Apart from being difficult to extract, these fragile cultures also had to pass through extremely harsh dissociation steps, and a risk of being...
Despite the significant efforts made in understanding of various aspects of cancer such as progression, metastasis, tumor microenvironment, we have achieved very limited clinical success. It is majorly because cancer is a heterogeneous disease having varied histopathology, genetic differences making difficult to understand disease mechanism as well as discover therapeutic agents for it. The heterogeneity in...
Pancreatic islets Pancreatic islets, also called islets of Langerhans, are groups of cells present in pancreas named after the the German physician Paul Langerhans, who first described them in 1869. The normal human pancreas contains about one million islets. Pancreas plays an essential role in converting the food into fuel for the body’s cells. The...
Experiments with primary cells are playing a major role in advancement of life science and pharmaceutical research. Primary cells are little difficult to work with, but these cells provide more meaningful data in experiments. Here are ten common mistakes that are made with primary cells and also how these mistakes can be minimized. These tips will enhance...
Cell culture technology is playing a major role in the advancement of modern biotechnology especially in the area of human health, medicine and pharmaceutical technology. Ideally, the cells used in cell culture systems should mimic those present in vivo, thus primary cells (non-immortalized cells that have recently been removed from an in vivo environment) have...
In vitro cell culture experiments play an important role in the advancement of life science and biomedical research. These cell culture studies have become an integral part of research related to diagnosis, drug development, and drug toxicity and add significant value to the understanding of cellular and functional aspects of physiology and metabolism. However, the in vitro...
The preclinical studies in life science research involve the use of animals which is time consuming, expensive and also leads to suffering of that animal. The environmentalists and animal lovers
For decades, cell lines are playing a major role in advancement of scientific research and cell-based technologies. Cell culture experiments are of much importance in research especially for human based projects, yet researchers have to be very cautious during interpretation of data generated from cell lines only.
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