Cryopreservation is an essential cell culture maintenance activity in biomedical labs to preserve cells for future use. There are various aspects of cryopreservation which need to be learnt and properly performed so as to obtain a good viable population of cells after thawing, in case a culture follows. Keeping these things in mind, we had...
Organoids are ex vivo model systems that revolutionized basic and translational cancer research in the domain of disease biology, since last decade. Organoid 3D cultures undergo mitogen-mediated proliferation as multicellular structures in semisolid matrices and while they possess some similarities like 3D spheroid cultures of cell lines, there are basic fundamental differences between them (Tsai...
Antibiotics have been a routinely used component in cell cultures to prevent the growth of bacteria but several researchers doing primary cell culture have showed their interest in avoiding them for several reasons in order to save their cultures and help the cells grow happy! Researchers unanimously agree on the saying, “With good lab practice,...
At the time of procuring new primary cells to start cell culture for research purposes, the decision of procuring fresh cells or frozen primary cells (cryopreserved cells) may not be a simple task. There are many factors that goes on to influence the decision, based oncell maintenance, cell type, cell functionality, and logistics. To help...
At the time of cell culture experiments, imaging stem cells in real-time can essentially provide crucial information on cell physiology but previously, there was no such technology without the risks of phototoxicity or the use of labeling dye agents. With holotomography technology, it is possible to perform 3D live-cell imaging of the stem cells under...
Cell culture started in the 1800s and with time, the trend of exploring new domains of cell culture technology to excel in biomedical and clinical research has been expanding into newer domains. Although 2020 has majorly focused the utilization of cell culture in COVID-19 diagnosis and drug development research besides playing an essential role in...
In vitro cell culture systems have been considered as essential research tools for biomedical and clinical research and development. Although it started with 2D cell culture consisting of cell monolayers, the recent practice of cell culture, specifically for primary cell culture, advanced 3D in vitro culture systems are widely being adopted for the advantages of...
Primary cell cultures refer to the removal of tissue from an organism and maintaining them in an appropriate medium to allow their division. One approach involves placing a piece of tissue in a glass or plastic vessel followed by bathing it in a medium. Division and growth of cells occur when cells separate from the...
With an increasing need for extensive research on SARS-CoV2 biology, the diagnosis of COVID-19 infections by cell culture relies heavily on the careful and aseptic collection of specimens. This is where specialized Viral Transport Medium (VTM) is required to protect the virus from environmental damage and maintain its activity with adequate sustainable transport system. Over...
Drug discovery and development extensively drives biomedical research and primary cell culture has been a very important turning point for the drug development domain. Along with primary cell culture, a critical factor in drug discovery is the study of biomarkers. The quality of biomarker detection encourages and enhances the efficiency of drug target identification, biological...
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