The task of choosing cell culture media for use with your primary cells is surely daunting. Therefore, if you are thinking of replicating a formulation of media from any previously published report or if you are optimizing your nutrient or classical media, it is important to understand the consequences of this media choice for your...
As per the central dogma, protein production by translation is possible after RNA transcription and it all begins with DNA replication. In simpler terms, DNA sequence provides instructions for protein synthesis, and thus a gene expression results in protein expression. The recombinant DNA technology, also known as DNA cloning or gene cloning, involves the transfer...
In the field of disease biology, cell therapy, and developmental biology, the experimental approaches do not result in reproducible efficiency upon using cell lines as limitations of genetic difference and characterization morphing occur in such cases. Therefore, scientists have shifted towards using primary cells and stem cells for in vitro cell culture studies in order...
CAR-T cell therapy is a type of treatment where the patient’s T cells are modified in the laboratory for attacking the cancer cells. This type of modification involves adding the gene for a special receptor that binds to a certain cancer cell protein. The special receptor is named CAR or chimeric antigen receptor. CAR-T cell...
Cryopreservation has become one of the major steps to focus on enhancing commercial and clinical cellular experiment-based cell culture process. Non-optimized substandard protocols and reagents used in cell culture can lead to damage of cells and lower cell viability. Besides that, the lack of optimized protocols in cell culture practice might also lead to decreased...
Primary cell culture is an expanding technology for several biomedical applications like tissue culture, 3D Cell Culture, bioscaffolds, 3D bioprinting, organoid culture etc. Researchers have been utilizing this technology as an efficient ethical alternative to animal experiments and as a physiologically in vivo-mimicking alternative to culturing continuous cell lines. We, at Kosheeka, believe that cell...
If you are a cell culture researcher working with primary cell culture, the most difficult thing to accept is watching your cell dishes and flasks getting contaminated or not proliferating even with appropriate incubation at the correct media pH and temperature conditions. Watching those adherent cells not attached to the flask surface and knowing that...
Cryopreservation is an essential cell culture maintenance activity in biomedical labs to preserve cells for future use. There are various aspects of cryopreservation which need to be learnt and properly performed so as to obtain a good viable population of cells after thawing, in case a culture follows. Keeping these things in mind, we had...
Organoids are ex vivo model systems that revolutionized basic and translational cancer research in the domain of disease biology, since last decade. Organoid 3D cultures undergo mitogen-mediated proliferation as multicellular structures in semisolid matrices and while they possess some similarities like 3D spheroid cultures of cell lines, there are basic fundamental differences between them (Tsai...
Antibiotics have been a routinely used component in cell cultures to prevent the growth of bacteria but several researchers doing primary cell culture have showed their interest in avoiding them for several reasons in order to save their cultures and help the cells grow happy! Researchers unanimously agree on the saying, “With good lab practice,...
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