Cryopreservation is a well-known laboratory technique for storing cells as well as other biological material at temperatures close to liquid nitrogen (-196°C). It offers the researcher a standby in case developing cells are damaged due to contamination, and it helps to minimize the incidence of genetic drift by permitting early passage cells to be used...
Category: <span>Cell Culture</span>
Antibiotics in Cell Culture: Friend or Foe?
Cell culture is a realm within itself. The medium in the culture dish includes nutrients that cells require to live and thrive. But, every once in a while, uninvited invaders breach the cultures, endangering the delicate stability of the system and tainting the scientific conclusions. Consequently, the cultures are futile and must be eradicated. But...
Organoids- Advanced 3D Cell Culture
The whole process of obtaining human tissue for studying organ development and diseases is tedious due to ethical concerns and difficulty of availability. 3D cell culture offers a unique alternative for advanced research on human tissue. 3D cell culture, in the form of organoids, is used by biomedical researchers to study the complex arrangements and...
Animal Cell Culture- Safety and Handling Considerations
A crucial step before taking up research work with human or animal tissue is to ensure appropriate ethical and medical legislation and guidelines for experiments. This can also deal with procuring approval from relevant authorities or individuals. Biohazards and Safety considerations It is very important to know the possible associated risks while working with potential...
Tips for Liver Hepatocyte Thawing for Cell Culture
Liver hepatocytes in cryopreservation contain major drug-metabolizing enzymes. For growing liver hepatocyte cell culture, a quick-thaw protocol is performed in the case of cryopreserved primary cells. However, if the thawing procedure is incorrect, the viability and functionality of the primary cells might be impaired. Inhibit Cryoinjury effects Before going for liver hepatocyte thawing, cryoinjury phenomena...
How Primary Cells Are Important for In Vitro ADMET?
ADME is the abbreviation for Absorption, Distribution, Metabolism, and Excretion. These ADME studies are designed to investigate the metabolism and processing of a chemical compound by a living organism. When toxicology tests are a part of this process, the acronym becomes ADMET with the last T for ‘Toxicology’. Primary cells are directly isolated from human...
Applications of Primary Cell Culture in Research
Cell culture is a prominent tool in cellular and molecular biology research, often spreading to clinical applications. Cell culture using primary cells can be excellent model systems for studying physiology and biochemistry of cells, drug toxicity and metabolism, and other biomedical applications. Primary cell culture is being used by researchers worldwide for their role in...
Advantages and Disadvantages of Using Primary Cells in Cell Culture
Primary cells represent the in vivo tissue environment fairly and these cells are directly taken from body tissues for processing and established them under optimized culture conditions. As these cells are derived directly from native body tissue and not modified, they mimic the in vivo state and physiology. Therefore, they provide excellent model systems for...
10 Amazing Facts About Cell Culture You Should Not Miss
Biomedical and clinical research would be inconceivable without progress in cell culture. This tool has become inevitably crucial in life sciences and has evolved much since the early 20th century, with its application in primary cell culture, 3D culture, tissue engineering, 3D bioprinting, and regenerative therapies. As a cell culture researcher, you must have enough...
2D vs 3D Cell Cultures – What’s The Difference?
In the field of biomedical research, 2D cell cultures have extensively been used since the early 1900s but in recent times, the technology of 3D cell culture has boomed. The tussle of 2D vs 3D cell culture has received much interest from biomedical researchers and other science geeks. In this article, let us explore more...