The task of choosing cell culture media for use with your primary cells is surely daunting. Therefore, if you are thinking of replicating a formulation of media from any previously published report or if you are optimizing your nutrient or classical media, it is important to understand the consequences of this media choice for your...
As per the central dogma, protein production by translation is possible after RNA transcription and it all begins with DNA replication. In simpler terms, DNA sequence provides instructions for protein synthesis, and thus a gene expression results in protein expression. The recombinant DNA technology, also known as DNA cloning or gene cloning, involves the transfer...
In the field of disease biology, cell therapy, and developmental biology, the experimental approaches do not result in reproducible efficiency upon using cell lines as limitations of genetic difference and characterization morphing occur in such cases. Therefore, scientists have shifted towards using primary cells and stem cells for in vitro cell culture studies in order...
CAR-T cell therapy is a type of treatment where the patient’s T cells are modified in the laboratory for attacking the cancer cells. This type of modification involves adding the gene for a special receptor that binds to a certain cancer cell protein. The special receptor is named CAR or chimeric antigen receptor. CAR-T cell...
Cryopreservation has become one of the major steps to focus on enhancing commercial and clinical cellular experiment-based cell culture process. Non-optimized substandard protocols and reagents used in cell culture can lead to damage of cells and lower cell viability. Besides that, the lack of optimized protocols in cell culture practice might also lead to decreased...
Primary cell culture is an expanding technology for several biomedical applications like tissue culture, 3D Cell Culture, bioscaffolds, 3D bioprinting, organoid culture etc. Researchers have been utilizing this technology as an efficient ethical alternative to animal experiments and as a physiologically in vivo-mimicking alternative to culturing continuous cell lines. We, at Kosheeka, believe that cell...
If you are a cell culture researcher working with primary cell culture, the most difficult thing to accept is watching your cell dishes and flasks getting contaminated or not proliferating even with appropriate incubation at the correct media pH and temperature conditions. Watching those adherent cells not attached to the flask surface and knowing that...
Mouse models for inflammatory bowel disease (IBD) are of various types and subtypes as the different models have different responses of histology, disease location, therapeutic response, and potential applications. Chemically Induced Mouse Models Chemically induced mouse models are the most widely used inflammatory bowel disease study moels. These mouse models are chemically induced by dextran sulfate...
In recent times, PDX models or patient-derived xenograft models are getting heavily utilized in head and neck oncology research, specifically for studies on radiation response and organoids. What is a Patient-Derived Xenograft? Patient-derived xenografts (PDX) are xenograft models that have been derived from patient tumor tissues but never adapted to grow like cell line-derived xenografts. Patient-derived...
Preclinical research models of cancer, such as human tumor xenografts, murine tumor homografts, cell line models and 3D organoid models, are extensively used in research and development for oncology studies. Since these tumor models are used for a wide spectrum of research applications, they are often utilized globally but the cell lines which are used...
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