Primary Cells

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Culture of Primary Cells

Primary cells are grown after isolation from specific animal tissues. The tissue extracts undergo disaggregation by enzymatic process and are incubated in grown medium for proliferation. Following that, these cells are further purified and separated according to the required cell lineage.

Application of Primary Cells

Drug screening and toxicity evaluation

Primary cells have a wide application in the pharmaceutical industry to evaluate drug dosage and toxicity.

In vitro model for Cell Biology research

Primary cells are excellent in vitro models to study cell biology, biochemical assays, and metabolism.

A substitute for animal studies

Primary cells in 3D cell culture models can bypass the ethical regulations of animal experiments.

Tissue Engineering and Regenerative medicine

Primary cells have extensively applications in tissue engineering and regenerative medicine.

Advantages of Primary Cells

Closely mimics in vivo conditions

Primary cells closely stimulate biological living model thus provide significant and high quality results in experiments

Minimal chromosomal aberrations

The Major disadvantage with established cell line is that after a period, cell characteristics may change and become quite different from initial passages. Generally primary cells not only retain normal physiology but also genetic constitution when propagated in culture.

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